Coenzyme-dependent nanozymes playing dual roles in oxidase and reductase mimics with enhanced electron transport. Issue 46 (23rd November 2020)

• Record Type: Journal Article
• Authors: Chen, Jinxing; Ma, Qian; Li, Minghua; Wu, Weiwei; Huang, Liang; Liu, Ling; Fang, Youxing; Dong, Shaojun
• Is Part Of: Nanoscale. Volume 12:Issue 46(2020)
• Page Start: 23578
• Page End: 23585
• Publication Date: 2020-11-23
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d0nr06605b
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : PEI/ZIF-FMN mediated the electron transfer from NADH to cytochrome c. Abstract : Although nanozymes overcome a series of shortcomings of natural enzymes, their wide applications are hampered due to their limited varieties. In this work, we propose a coenzyme-dependent nanozyme, a synergistic composite comprising zeolitic imidazolate frameworks encapsulated with polyethylenimine (PEI) and functionalized with a flavin mononucleotide (PEI/ZIF-FMN). The flavin mononucleotide (FMN) plays the role of a prosthetic group, and the positively charged NH2 groups in PEI readily provide the binding affinity to nicotinamide adenine dinucleotide (NADH), which facilitates the electron transfer from NADH to FMN and terminal electron acceptors (such as O2 ) with a greatly enhanced (80 times) catalytic performance. The integrated nanoparticle–coenzyme composite works as an NADH oxidase mimic and couples with dehydrogenases for the tandem enzymatic reaction. PEI/ZIF-FMN also mediated the electron transfer from NADH to cytochrome c (Cyt c), thereby exhibiting Cyt c reductase-like activity.