Multi-aperture Fourier ptychographic microscopy, theory and validation. (March 2021)

• Record Type: Journal Article
• Authors: Konda, Pavan Chandra; Taylor, Jonathan M.; Harvey, Andrew R.
• Is Part Of: Optics and lasers in engineering. Volume 138(2021)
• Publication Date: 2021-03
• Subjects: Fourier ptychography -- Aperture synthetic microscopy -- Phase retrieval; Lasers in engineering -- Periodicals; Optical measurements -- Periodicals; Optics -- Periodicals; Lasers en ingénierie -- Périodiques; Mesures optiques -- Périodiques; Optique -- Périodiques; 621.36605
• DOI: 10.1016/j.optlaseng.2020.106410
• Languages: English
• ISSNs: 0143-8166

Abstract

Highlights: We present a multi-camera-based method for improving data capture speed in Fourier ptychographic microscopy. A new reconstruction algorithm along with robust calibration methods are proposed for multi-camera Fourier ptychographic systems. A proof-of-concept experiment is performed to validate the principle by imaging a resolution target and a plant root cross section, using 9 times fewer LED illuminations compared to a regular Fourier ptychographic setup. Abstract: We present a novel microscopy concept, termed Multi-Aperture Fourier ptychographic microscopy (MA-FPM), to enable implementation of parallel detectors in microscopy to increase the space-bandwidth-time product. MA-FPM is a synthetic aperture technique: an array of objectives together with tilt-shift illumination are used to synthesize high-resolution, wide field-of-view images. Here, the phase is recovered using Fourier ptychography (FP) algorithms unlike conventional optical synthetic aperture techniques where holographic measurements are used. In this article we report a proof-of-concept experiment by translating a lens and a detector to positions according to the proposed design and demonstrate high-quality imaging performance despite using nine-fold fewer illumination angles compared to an equivalent FP setup. Calibration procedures and a reconstruction algorithm were developed to address the challenges of multiple imaging systems.