Enhancement of polychlorinated biphenyl biodegradation by resuscitation promoting factor (Rpf) and Rpf-responsive bacterial community. (January 2021)

• Record Type: Journal Article
• Authors: Su, Xiaomei; Li, Si; Xie, Mengqi; Tao, Linqin; Zhou, Yan; Xiao, Yeyuan; Lin, Hongjun; Chen, Jianrong; Sun, Faqian
• Is Part Of: Chemosphere. Volume 263(2021)
• Publication Date: 2021-01
• Subjects: Aroclor 1242 -- Rpf protein -- Biodegradation -- Bacterial community -- Uncultured bacteria; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2020.128283
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: The activities of indigenous bacterial communities in polychlorinated biphenyls (PCBs) contaminated environments is closely related to the efficiency of bioremediation processes. Using resuscitation promoting factor (Rpf) from Micrococcus luteus is a promising method for resuscitation and stimulation of functional bacterial populations under stressful conditions. This study aims to use the Rpf to accelerate the biodegradation of Aroclor 1242, and explore putative PCB degraders which were resuscitated by Rpf addition. The Rpf-responsive bacterial populations were investigated using culture-dependent and culture-independent approaches, respectively. The results confirm that Rpf was capable of enhancing PCB degradation of enriched cultures from PCB-contaminated soils, and improving the activities of cultures with low tolerance to PCBs. High-throughput 16S rRNA analysis displays that the Rpf greatly altered the composition and abundance of bacterial populations in the phylum Proteobacteria . Identification of the resuscitated strains further suggests that the Rpf-responsive population was mostly represented by Sphingomonas and Pseudomonas, which are most likely the key PCB-degraders for enhanced biodegradation of PCBs. Graphical abstract: Image 1 Highlights: Rpf accelerated biodegradation of PCBs and growth rate of functional population. PCB degradation efficiencies of Rpf treatments was 5.4%–35.1% higher than controls. Stimulation function of Rpf linked mainly to PCB degraders within Proteobacteria. Rpf-responsive resuscitated strains predominated by Sphingomonas and Pseudomona s.