Evaluation of a biosurfactant producing bacterial strain Pseudomonas sp. ISTPY2 for efficient pyrene degradation and landfill soil bioremediation through soil microcosm and proteomic studies. (December 2020)

• Record Type: Journal Article
• Authors: Swati, ; Kumari, Moni; Ghosh, Pooja; Thakur, Indu Shekhar
• Is Part Of: Bioresource technology reports. Volume 12(2020)
• Publication Date: 2020-12
• Subjects: Soil Microcosm -- Pyrene Biodegradation -- Pseudomonas sp. -- Ghazipur Landfill -- Proteins -- GC‐MS; Biomass energy -- Periodicals; Biotransformation (Metabolism) -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Waste products as fuel -- Periodicals; Waste products as fuel; Organic wastes; Factory and trade waste; Biotransformation (Metabolism); Biomass energy; Agricultural wastes; Periodicals; Electronic journals; 662.88
• DOI: 10.1016/j.biteb.2020.100607
• Languages: English
• ISSNs: 2589-014X

Abstract

Abstract: Landfill soil pollution is a huge cause of concern particularly in the developing countries like India where unsegregated wastes are disposed off in the open dumping sites, posing a high health risk to workers and rag pickers. This necessitates an efficient strategy for landfill soil reclamation. The present study evaluates the efficiency of a biosurfactant producing strain Pseudomonas sp. ISTPY2 isolated from landfill soil for pyrene degradation and landfill soil remediation in a soil microcosm. The metabolites were identified using GC–MS and an increase in the number of aliphatic compounds was observed during the process with phthalic acid and alkanes as major metabolites. Proteomic study was performed to study the enzymes involved in pyrene degradation and it was found that various xenobiotic degrading proteins such as phthalate 4, 5-dioxygenase, aldehyde dehydrogenase, F420-dependent oxidoreductase and others were induced in response to pyrene stress and played a role in its efficient biodegradation. Graphical abstract: Unlabelled Image Highlights: Pseudomonas sp. ISTPY2 can degrade wide range of pollutants found in landfill soil. Pseudomonas sp. ISTPY2 can degrade upto 97% of pyrene in soil microcosm. The xenobiotic degrading proteins were detected in pyrene induced sample. Pseudomonas sp. ISTPY2 can be utilized for bioremediation of landfill soil.