Biofunctionalized nanodot zirconia-based efficient biosensing platform for noninvasive oral cancer detection. (December 2020)

• Record Type: Journal Article
• Authors: Kumar, Suveen; Chauhan, Dipti; Renugopalakrishnan, Venkatesan; Malhotra, Bansi D.
• Is Part Of: MRS communications. Volume 10:Number 4(2020)
• Page Start: 652
• Page End: 659
• Publication Date: 2020-12
• Subjects: Materials -- Periodicals; 620.11
• DOI: 10.1557/mrc.2020.75
• Languages: English
• ISSNs: 2159-6859

Abstract

Abstract : Abstract: The authors report results of the studies relating to the synthesis of nanodot zirconia that has been utilized for the fabrication of electrochemical biosensing platform for the detection of CYFRA-21-1 biomarker, secreted in saliva samples of oral cancer patients. For the synthesis of nanodot zirconia (ndZrO2 ), the hydrothermal process was used and further functionalized with 3-aminopropyl triethoxysilane (APTES). Electrophoretic deposition technique was employed for its deposition onto the ITO electrode. The EDC-NHS reaction was used for anti-CYFRA-21-1 immobilization and bovine serum albumin (BSA) was used for blocking of the nonspecific binding sites. The fabricated biosensing platform (BSA/anti-CYFRA-21-1/APTES/ndZrO2 /ITO) exhibited a wide linear detection range (0.5–50 ng/mL) with excellent sensitivity (0.53 μA mL/ng cm 2 ).