Cancer-associated fibroblast regulation by microRNAs promotes invasion of oral squamous cell carcinoma. (November 2020)

• Record Type: Journal Article
• Authors: Matos, Leandro Luongo; Menderico Junior, Gilberto Mendes; Theodoro, Thérèse Rachell; Pasini, Fatima Solange; Ishikawa, Marina de Menezes; Ribeiro, Andrômeda Aryane Bomtempo; de Mello, Evandro Sobroza; Pinhal, Maria Aparecida da Silva; Moyses, Raquel Ajub; Kulcsar, Marco Aurelio Vamondes; Dedivitis, Rogério Aparecido; Cernea, Claudio Roberto; Kowalski, Luiz Paulo
• Is Part Of: Oral oncology. Volume 110(2020)
• Publication Date: 2020-11
• Subjects: Carcinoma, squamous cell -- Mouth -- microRNAs -- Neoplasm invasiveness -- Carcinogenesis; Mouth -- Cancer -- Periodicals; Mouth -- Tumors -- Periodicals; Mouth Diseases -- Periodicals; Mouth Neoplasms -- Periodicals; Bouche -- Cancer -- Périodiques; Bouche -- Tumeurs -- Périodiques; Tumeurs -- Périodiques; Electronic journals; 616.9943105
• DOI: 10.1016/j.oraloncology.2020.104909
• Languages: English
• ISSNs: 1368-8375

Abstract

Highlights: There is interaction between miRNAs and the extracellular matrix in OSCC. miR-1-3p, miR-133a-3 and miR-21-5p are involved in the process of OSCC invasion. MMP-2 and MMP-9 in CAFs are related to ECM degradation in deep tumors. miR-133a-3p expression could be related to OS and DFS in OSCC. Abstract: The objective of the present study was to evaluate the role of microRNA-mediated remodeling of the extracellular matrix in the process of tumor invasion of oral squamous cell carcinoma and to evaluate its relationship with the prognosis of these patients. This was a retrospective study on material from the paraffin blocks of patients operated on for oral squamous cell carcinoma, in addition to a group of healthy oral mucosa samples of paired patients. miR-1-3p, miR-133-3p, and miR-21-5p were differentially expressed between the superficial and deep tumor groups. miR-21-5p was the one with the greatest accuracy in the differentiation between superficial and deep tumors. By immunohistochemistry, the group of deep tumors showed greater immunoreactivity to matrix metalloproteinases 2 and 9 and laminin α in tumor-associated fibroblasts, with consequent degradation of the basal membrane, measured by greater loss of continuity of type IV collagen. This process was also associated with lower and higher expression of miR-1-3p and miR-21-5p, respectively. There was also a trend toward better overall and disease-free survival rates in patients with higher miR-133a-3p. The present study showed the interaction between microRNAs and extracellular matrix remodeling in oral squamous cell carcinoma.