Sequential dual site-selective protein labelling enabled by lysine modification. Issue 22 (15th November 2020)

• Record Type: Journal Article
• Authors: Matos, Maria J.; Brown, Libby; Bernardim, Barbara; Guerreiro, Ana; Jiménez-Osés, Gonzalo; Bernardes, Gonçalo J.L.
• Is Part Of: Bioorganic & medicinal chemistry. Volume 28:Issue 22(2020)
• Publication Date: 2020-11-15
• Subjects: Protein modification -- Bioorthogonal labelling -- Lysine modification -- Albumin -- Antibodies; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2020.115783
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins.