Automated Single-Particle Reconstruction of Heterogeneous Inorganic Nanoparticles. (December 2020)
- Record Type:
- Journal Article
- Title:
- Automated Single-Particle Reconstruction of Heterogeneous Inorganic Nanoparticles. (December 2020)
- Main Title:
- Automated Single-Particle Reconstruction of Heterogeneous Inorganic Nanoparticles
- Authors:
- Slater, Thomas J. A.
Wang, Yi-Chi
Leteba, Gerard M.
Quiroz, Jhon
Camargo, Pedro H. C.
Haigh, Sarah J.
Allen, Christopher S. - Abstract:
- Abstract: Abstract : Single-particle reconstruction can be used to perform three-dimensional (3D) imaging of homogeneous populations of nano-sized objects, in particular viruses and proteins. Here, it is demonstrated that it can also be used to obtain 3D reconstructions of heterogeneous populations of inorganic nanoparticles. An automated acquisition scheme in a scanning transmission electron microscope is used to collect images of thousands of nanoparticles. Particle images are subsequently semi-automatically clustered in terms of their properties and separate 3D reconstructions are performed from selected particle image clusters. The result is a 3D dataset that is representative of the full population. The study demonstrates a methodology that allows 3D imaging and analysis of inorganic nanoparticles in a fully automated manner that is truly representative of large particle populations.
- Is Part Of:
- Microscopy and microanalysis. Volume 26:Number 6(2020)
- Journal:
- Microscopy and microanalysis
- Issue:
- Volume 26:Number 6(2020)
- Issue Display:
- Volume 26, Issue 6 (2020)
- Year:
- 2020
- Volume:
- 26
- Issue:
- 6
- Issue Sort Value:
- 2020-0026-0006-0000
- Page Start:
- 1168
- Page End:
- 1175
- Publication Date:
- 2020-12
- Subjects:
- electron tomography, -- nanoparticles, -- scanning transmission electron microscopy, -- three-dimensional imaging
Microscopy -- Periodicals
Microchemistry -- Periodicals
502.82 - Journal URLs:
- https://academic.oup.com/mam ↗
http://journals.cambridge.org/action/displayJournal?jid=MAM ↗
http://link.springer.de/link/service/journals/10005/index.htm ↗
http://firstsearch.oclc.org ↗ - DOI:
- 10.1017/S1431927620024642 ↗
- Languages:
- English
- ISSNs:
- 1431-9276
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD Digital store
- Ingest File:
- 14890.xml