Amperometric Detection of Creatinine in Clinical Samples Based on Gold Electrode Arrays Fabricated Using Printed Circuit Board Technology. Issue 12 (3rd November 2020)

• Record Type: Journal Article
• Authors: Ortiz, Mayreli; Botero, Mary Luz; Fragoso, Alex; O'Sullivan, Ciara K.
• Is Part Of: Electroanalysis. Volume 32:Issue 12(2020)
• Page Start: 3054
• Page End: 3059
• Publication Date: 2020-11-03
• Subjects: Creatinine detection -- printed board circuit -- enzymatic biosensor -- amperometric detection; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202060446
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Herein we report a reliable and stable biosensor for the amperometric detection of creatinine through the ammonium produced in its reaction with creatinine deiminase linked to a Nafion®/polyaniline gold coated surface. The gold electrode array was produced using a low cost printed board circuit technology. A linear range of 1–1000 μM was obtained with standard deviations of less than 5 % (n=3) obtained. Stability studies were carried out and 80 % activity was maintained after one month. The sensors were tested with 142 serum samples from patients with renal dysfunction at different stage of the disease and validated using a commercial colorimetric method. Abstract :