Combined effects of maternal exposure to fungicides on behavioral development in F1‐generation mice: 2. Fixed‐dose study of thiabendazole. Issue 20 (5th September 2020)

• Record Type: Journal Article
• Authors: Tanaka, Toyohito; Suzuki, Toshinari; Inomata, Akiko; Moriyasu, Takako
• Is Part Of: Birth defects research. Volume 112:Issue 20(2020)
• Page Start: 1809
• Page End: 1824
• Publication Date: 2020-09-05
• Subjects: behavioral development -- enilconazole -- fungicide -- imazalil -- mice -- maternal exposure -- movement activity -- reproductive toxicity -- spontaneous behavior -- thiabendazole; Teratology -- Periodicals; Abnormalities, Human -- Periodicals; Congenital Abnormalities; Embryo, Mammalian -- abnormalities; Teratology; Abnormalities, Human; Teratology; Periodicals; Periodicals; 616.043
• DOI: 10.1002/bdr2.1793
• Languages: English
• ISSNs: 2472-1727

Abstract

Abstract: Background: Few published studies are reported for the neurobehavioral toxicity of combined exposure to fungicides in mammals. This study was aimed to re‐evaluate the reproductive and neurobehavioral effects of maternal exposure to combined imazalil (IMZ) and thiabendazole (TBZ) with fixed‐dose of TBZ in mice. Methods: IMZ/TBZ were given in the diet to provide levels of 0%/0% (control), 0.0015%/0.018% (IMZ/TBZ), 0.006%/0.018% and 0.024%/0.018% during the gestation and lactation periods. Selected reproductive and neurobehavioral parameters were measured in the F1 generation. Results: No adverse effect of IMZ/TBZ was observed in litter size, litter weight, or sex ratio at birth. Concerning behavioral developmental parameters, the cliff avoidance on PND 7 of male offspring was restrained significantly in the treatment groups in a dose‐related manner. Exploratory behavior examination indicated that the average time of rearing significantly lengthened in the high‐dose group of male offspring. After weaning, the average time of rearing in exploratory behavior lengthened in a significant dose‐related trend in adult females of the F1 generation. Spontaneous behavior examination indicated that the average speed decreased significantly through 120 min in the high‐dose group of the F1 ‐generation males. In females, the average time of rearing lengthened significantly through 120 min in the high‐dose group. In the longitudinal patterns, the parallel lines of the control and treatment groups indicated a significant distance in the average time of rearing in the F1 ‐generation females. Conclusions: The results from two combined exposure studies of IMZ/TBZ suggest that TBZ concentrations have caused major effects on exploratory and spontaneous behavior.