Multi‐drug resistant, biofilm‐producing high‐risk clonal lineage of Klebsiella in companion and household animals. (11th September 2020)

• Record Type: Journal Article
• Authors: Banerjee, A.; Batabyal, K.; Singh, A.D.; Joardar, S.N.; Dey, S.; Isore, D.P.; Sar, T.K.; Dutta, T.K.; Bandyopadhyay, S.; Samanta, I.
• Is Part Of: Letters in applied microbiology. Volume 71:Number 6(2020)
• Page Start: 580
• Page End: 587
• Publication Date: 2020-09-11
• Subjects: companion animals -- ESBL -- goats -- Klebsiella -- sheep; Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.13381
• Languages: English
• ISSNs: 0266-8254

Abstract

Abstract: Antimicrobial resistance is a global emergency which needs one health approach to address. The present study was conducted to detect the prevalence of beta‐lactamase and biofilm‐producing Klebsiella strains in rectal swabs ( n = 624) collected from healthy dogs, cats, sheep and goats reared as companion or household animals in India. The dogs and cats were frequently exposed to third‐ or fourth‐generation cephalosporins for therapy. The sheep and goats were occasionally exposed to antibiotics and had environmental exposure. Phenotypical ESBL ( n = 93) and ACBL ( n = 88)‐producing Klebsiella were isolated significantly more ( P < 0·05) from companion animals than household animals. Majority of the Klebsiella possessed bla CTX‐M‐15 . The sequences bla CTX‐M‐15.2, bla CTX‐M‐197 and bla CTX‐M‐225 are reported first time from the companion animals. All ACBL‐producing isolates possessed bla AmpC . The present study detected 65·8% of Klebsiella strains as biofilm producers possessing the studied biofilm associated genes. The isolates showed phenotypical resistance against chloramphenicol, tetracycline, doxycycline, co‐trimoxazole, ampicillin, cefotaxime/clavulanic acid. The present study showed that companion and household animals (dogs, cats, sheep, goats) may act as a carrier of ESBL/biofilm‐producing, multi‐drug resistant, high‐risk clonal lineage of Klebsiella . Abstract : Significance and Impact of the Study: Characterization of antimicrobial resistance is an utmost necessity in animals having direct contact with human as a part of the one health approach. This is the first large‐scale monitoring of ESBL/biofilm‐producing Klebsiella in companion/household animals in India sharing human habitat. Majority of the Klebsiella possessed blaCTX‐M‐15 which is associated with infection in both humans and animals throughout the world. Few nucleotide sequences of blaCTX‐M in the present study are reported for the first time from the companion animals. The emergence of resistance determinants in Klebsiella i solated from companion and household animals was correlated with therapeutic antibiotic exposure and contaminated environment, respectively.