Dual-recognition-based determination of ctDNA via the clamping function of peptide nucleic acid and terminal protection of small-molecule-linked DNA. Issue 23 (29th September 2020)

• Record Type: Journal Article
• Authors: Chen, Chaohui; He, Rongxiang; Zhang, Zhengtao; Chen, Yong
• Is Part Of: Analyst. Volume 145:Issue 23(2020)
• Page Start: 7603
• Page End: 7608
• Publication Date: 2020-09-29
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an01305f
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A new dual-recognition fluorescent biosensor for circulating tumor DNA (ctDNA) detection has been developed, which combines the clamping function of peptide nucleic acid (PNA) and terminal protection of small-molecule-linked DNA (TPSMLD). Abstract : A new dual-recognition fluorescent biosensor for circulating tumor DNA (ctDNA) detection has been developed, which combines the clamping function of peptide nucleic acid (PNA) and terminal protection of small-molecule-linked DNA (TPSMLD). Taking the tumor-specific E542K mutation and methylation of the PIK3CA gene as the target ctDNA, a low detection limit of 0.3161 pM ctDNA is achieved with good selectivity. This study not only offers a sensitive, selective and accurate ctDNA detection method, but can also be used to detect the target in complex biological samples.