Integrating nucleic acid sequence-based amplification and microlensing for high-sensitivity self-reporting detection. Issue 23 (23rd September 2020)

• Record Type: Journal Article
• Authors: Teng, Feiyue; Wu, Xinpei; Hong, Tao; Munk, Gary B.; Libera, Matthew
• Is Part Of: Analyst. Volume 145:Issue 23(2020)
• Page Start: 7528
• Page End: 7533
• Publication Date: 2020-09-23
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d0an01231a
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : We use electron-beam patterned functional microgels to integrate self-reporting molecular beacons, dielectric microlenses, and solid-phase and/or solution-phase nucleic acid amplification in a viral-detection microarray model. Abstract : We use electron-beam patterned functional microgels to integrate self-reporting molecular beacons, dielectric microlenses, and solid-phase and/or solution-phase nucleic acid amplification in a viral-detection microarray model. The detection limits for different combinations of these elements range from 10 −10 M for direct target-beacon hybridization alone to 10 −18 M when all elements are integrated simultaneously.