MIR22HG regulates miR-486/PTEN axis in bladder cancer to promote cell proliferation. Issue 6 (23rd June 2020)

• Record Type: Journal Article
• Authors: Tang, Qisheng; Jiang, Xue; Ma, Shanjin; Wang, Lei; Li, Ruixiao; Ma, Jianjun
• Is Part Of: Bioscience reports. Volume 40:Issue 6(2020)
• Publication Date: 2020-06-23
• Subjects: bladder cancer -- miR-486 -- MIR22HG -- proliferation -- PTEN; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1042/BSR20193991
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract: The tumor suppressive role of MIR22HG has been studied in several types of cancer. We analyzed the TCGA dataset and found the down-regulation of MIR22HG in bladder cancer (BC). Bioinformatics analysis predicted the interaction between MIR22HG and miR-486. The direct interaction between MIR22HG and miR-486 was also confirmed by dual luciferase assay. However, overexpression of these two factors did not significantly affect the expression of each other. Interestingly, overexpression of MIR22HG led to up-regulated phosphatase and tensin homolog (PTEN), which is a target of miR-486. In cell proliferation assay, overexpression of MIR22HG and PTEN led to decreased rates of BC cell proliferation. Moreover, overexpression of miR-486 played an opposite role and attenuated the effects of overexpression of MIR22HG and PTEN. Therefore, MIR22HG regulates miR-486/PTEN axis to promote cell proliferation in BC.