Structural and functional insights into macrophage migration inhibitory factor from Oncomelania hupensis, the intermediate host of Schistosoma japonicum. Issue 12 (22nd June 2020)

• Record Type: Journal Article
• Authors: Su, Zhiming; Tian, Xuyang; Li, Huanjun; Wei, Zhiming; Chen, Lifan; Wang, Songqing; Ren, Haixia; Peng, Wenfeng; Tang, Chongti; Lin, Tianwei; Huang, Shuaiqin
• Is Part Of: Biochemical journal. Volume 477:Issue 12(2020)
• Page Start: 2133
• Page End: 2151
• Publication Date: 2020-06-22
• Subjects: crystal structure -- functional characteristics -- MIF cytokine -- Oncomelania hupensis; Biochemistry -- Periodicals; 572
• DOI: 10.1042/BCJ20200068
• Languages: English
• ISSNs: 0264-6021

Abstract

Abstract : Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum . As an irreplaceable prerequisite in the transmission and prevalence of schistosomiasis japonica, an in-depth study of this obligate host–parasite interaction can provide glimpse into the molecular events in the competition between schistosome infectivity and snail immune resistance. In previous studies, we identified a macrophage migration inhibitory factor (MIF) from O. hupensis ( Oh MIF), and showed that it was involved in the snail host immune response to the parasite S. japonicum . Here, we determined the crystal structure of Oh MIF and revealed that there were distinct structural differences between the mammalian and O. hupensis MIFs. Noticeably, there was a projecting and structured C-terminus in Oh MIF, which not only regulated the MIF's thermostability but was also critical in the activation of its tautomerase activity. Comparative studies between Oh MIF and human MIF (hMIF) by analyzing the tautomerase activity, oxidoreductase activity, thermostability, interaction with the receptor CD74 and activation of the ERK signaling pathway demonstrated the functional differences between hMIF and Oh MIF. Our data shed a species-specific light on structural, functional, and immunological characteristics of Oh MIF and enrich the knowledge on the MIF family.