MiR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer. Issue 9 (2nd September 2020)

• Record Type: Journal Article
• Authors: Wang, Hui; Hu, Hui; Luo, Zhenzhao; Liu, Shuiyi; Wu, Wangze; Zhu, Man; Wang, Jing; Liu, Yingle; Lu, Zhongxin
• Is Part Of: Bioscience reports. Volume 40:Issue 9(2020)
• Publication Date: 2020-09-02
• Subjects: α/β-hydrolase domain-containing 2 -- Cervical Cancer -- HPV16 E6/E7 -- miR-4454 -- NUDT21; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1042/BSR20200796
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract: The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 are identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasion and migration, whereas, inhibit the apoptosis in CaSki and C33A cells.