Dietary taurine supplementation attenuates lipopolysaccharide-induced inflammatory responses and oxidative stress of broiler chickens at an early age. (18th September 2020)

• Record Type: Journal Article
• Authors: Han, Hongli; Zhang, Jingfei; Chen, Yanan; Shen, Mingming; Yan, Enfa; Wei, Chengheng; Yu, Caiyun; Zhang, Lili; Wang, Tian
• Is Part Of: Journal of animal science. Volume 98:Number 10(2020)
• Publication Date: 2020-09-18
• Subjects: broiler -- immunological stress -- lipopolysaccharide -- liver injury -- oxidative damage -- taurine; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/skaa311
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: This study was conducted to investigate the effect of taurine as a prophylactic treatment on antioxidant function and inflammatory responses of broilers challenged with lipopolysaccharide (LPS ). A total of 256 one-day-old male Arbor Acres broiler chicks were randomly assigned to four treatments with eight replicates of eight birds (eight birds per cage). Four treatment groups were designated as follows: 1) in the CON group, broilers fed a basal diet; 2) in the LPS group, LPS-challenged broilers fed a basal diet; 3) in the LPS + T1 group, LPS-challenged broilers fed a basal diet supplemented with 5.0 g/kg taurine; and 4) in the LPS + T2 group, LPS-challenged broilers fed a basal diet supplemented with 7.5 g/kg taurine. The LPS-challenged broilers were intraperitoneally injected with 1 mg/kg body weight (BW ) of LPS at 16, 18, and 20 d of age, whereas the CON group received an injection of sterile saline. The results showed that broilers injected with LPS exhibited decreased ( P < 0.05) the average daily gain (ADG ) and the 21-d BW ( P < 0.05), while taurine supplementation alleviated the negative effects of LPS. Additionally, the LPS-induced increases ( P < 0.05) in serum alanine transaminase and aspartate transaminase activities were reversed by taurine supplementation. The taurines could alleviate the hepatic oxidative stress, with the presence of lower content of malondialdehyde ( P < 0.05), higher content of glutathione ( P < 0.05), and an increased glutathione peroxidase (GSH-Px ) activity ( P < 0.05). The concentrations of interleukin-1β (IL-1β ), interleukin-6 (IL-6 ), and tumor necrosis factor-α (TNF-α ) in the liver were measured by ELISA kits, and the result showed that dietary taurine supplementation prevented these cytokines increases in the liver of LPS-induced broilers. Taurine reduced the genes expression of IL-1 β, TNF- α, IL-6, cyclooxygenase-2, and inducible nitric oxide synthase, whereas it boosted the expression levels of antioxidant-related genes (nuclear factor erythroid 2-related factor 2, heme oxygenase-1, glutamate-cysteine ligase catalytic subunit, and GSH-Px ) in the liver of LPS-induced broilers. In conclusion, dietary taurine supplementation in broilers mitigated LPS-induced defects in ADG, oxidative stress, and inflammatory responses.