Susceptibility of Pseudomonas aeruginosa veterinary isolates to Pbunavirus PB1‐like phages. (19th October 2020)

• Record Type: Journal Article
• Authors: Fujiki, Jumpei; Furusawa, Takaaki; Munby, Montgomery; Kawaguchi, Chika; Matsuda, Yumie; Shiokura, Yusei; Nakamura, Keisuke; Nakamura, Tomohiro; Sasaki, Michihito; Usui, Masaru; Iwasaki, Tomohito; Gondaira, Satoshi; Higuchi, Hidetoshi; Sawa, Hirofumi; Tamura, Yutaka; Iwano, Hidetomo
• Is Part Of: Microbiology and immunology. Volume 64:Number 11(2020)
• Page Start: 778
• Page End: 782
• Publication Date: 2020-10-19
• Subjects: Bacteriophage -- PB1‐like phage -- Pbunavirus -- phage therapy -- Pseudomonas aeruginosa; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12846
• Languages: English
• ISSNs: 0385-5600

Abstract

Abstract: In recent years, antimicrobial‐resistant Pseudomonas aeruginosa strains have increased in the veterinary field. Therefore, phage therapy has received significant attention as an approach for overcoming antimicrobial resistance. In this context, we isolated and characterized four Pseudomonas bacteriophages. Phylogenetic analysis showed that the isolated phages are novel Myoviridae Pbunavirus PB1‐like phages with ØR12 belonging to a different clade compared with the other three. These phages had distinct lytic activity against 22 P. aeruginosa veterinary isolates. The phage cocktail composed from the PB1‐like phages clearly inhibited the occurrence of the phage‐resistant variant, suggesting that these phages could be useful in phage therapy.