Evaluation of total phenol and flavonoid content and antimicrobial and antibiofilm activities of Trollius chinensis Bunge extracts on Streptococcus mutans. Issue 12 (14th July 2020)

• Record Type: Journal Article
• Authors: Jiang, Min; Yan, Li; Li, Ke‐ao; Ji, Zhi‐hong; Tian, Shu‐ge
• Is Part Of: Microscopy research and technique. Volume 83:Issue 12(2020)
• Page Start: 1471
• Page End: 1479
• Publication Date: 2020-07-14
• Subjects: antibiofilms -- confocal laser scanning microscopy -- Streptococcus mutans -- Trollius chinensis Bunge; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23540
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Dental caries is a chronic disease with multiple bacterial infections, Streptococcus mutans is the main cariogenic bacteria. Trollius chinensis Bunge is a common folk medicine in the Xinjiang area of China. In this study, we investigated the total flavonoid content and total phenol content in four types of T. chinensis Bunge extracts and the inhibitory effects of these extracts on S. mutans . Agar diffusion method was used to measure the inhibition zone diameters, and the minimum inhibitory concentration and minimum bactericidal concentration were determined by the twofold dilution method. Water extracts from T. chinensis Bunge and ethanol (30, 60, and 90%) extracts at different concentrations could significantly inhibit the growth of S. mutans . Among them, 30% ethanol extract exhibited the best antibacterial and antibiofilms effect. Biofilm research (crystal violet staining and CLSM) showed that 30% ethanol extract of T. chinensis Bunge plays an important role in inhibiting S. mutans growth and the number of biofilms. The results indicate that T. chinensis Bunge extract has good antibacterial and anti‐biofilm activity on S. mutans. It has the potential to be developed for the treatment of caries in clinical application. Abstract : The water extracts and ethanol (30, 60, and 90%) extracts of T. chinensis Bunge can effectively restrain the growth of S. mutans. Additionally, the 30% ethanol extract of T. chinensis Bunge has the strongest inhibitory effect on the growth of S. mutans. The 30% ethanol extract of T. chinensis Bunge can effectively inhibit the biofilm of S. mutans. Trollius chinensis Bunge can be used as an effective natural product in the treatment of oral caries.