Reduced ex vivo stimulated IL-6 response in infants randomized to fish oil from 9 to 18 months, especially among PPARG2 and COX2 wild types. (March 2015)

• Record Type: Journal Article
• Authors: Harsløf, Laurine B.S.; Damsgaard, Camilla T.; Andersen, Anders D.; Aakjær, Ditte L.; Michaelsen, Kim F.; Hellgren, Lars I.; Frøkiær, Hanne; Vogel, Ulla; Lauritzen, Lotte
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 94(2015:Mar.)
• Page Start: 21
• Page End: 27
• Publication Date: 2015-03
• Subjects: COX-2 cyclooxygenase-2 -- FA% percentage of fatty acids -- FO fish oil -- IgE immunoglobulin E -- IL interleukin -- IFNγ interferon-γ -- LCPUFA long-chain polyunsaturated fatty acid -- LPS lipopolysaccharide -- PPARγ peroxisome proliferator-activated receptor-γ -- PUFA polyunsaturated fatty acid -- RBC erythrocytes -- SNP single nucleotide polymorphism -- SO sunflower oil -- Th T-helper cell -- T-RFLP terminal restriction fragment length polymorphism -- WT wild-type; Long-chain n−3 polyunsaturated fatty acids -- Immune maturation -- Child nutrition -- Polymorphisms -- Microbiota; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2014.10.007
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: We investigated whether n −3 LCPUFA affected immune function in late infancy and explored effect-modification by single nucleotide polymorphisms (SNPs) and links to intestinal microbiota. Infants ( n =105) were randomized to fish oil (FO, 1.2 g/d n −3 LCPUFA) or sunflower oil (SO)-supplements from age 9–18 months. Immune function was assessed by ex vivo cytokine production in stimulated blood and plasma immunoglobulin E (IgE). We genotyped functional SNPs in PPARG2 and COX2 and analyzed fecal microbiota by 16S-rRNA terminal restriction fragment length polymorphism. FO compared to SO reduced Lactobacillus paracasei -stimulated IL-6 at 18 months ( P =0.03, n =104). This effect was most pronounced among infants wild-type for PPARG2-Pro12Ala and/or COX2-T8473C ( P <0.05). Predominant bacterial fragments were associated with 18 months IgE in all infants ( P =0.004) (bp100) and with IL-6 production among infants weaned before 9 months ( P =0.047) (bp102). Thus, FO reduced IL-6 in a genotype-modified manner. The microbiota was partly linked to IL-6 and IgE, not directly to FO.