Depth-resolved imaging of intercellular structures of large intestine with polarization-sensitive FFOCT. (February 2021)

• Record Type: Journal Article
• Authors: Liu, Siyu; Gao, Wanrong
• Is Part Of: Optics and lasers in engineering. Volume 137(2021)
• Publication Date: 2021-02
• Subjects: Tissue birefringence -- Intercellular structures -- Large intestine -- Full-field optical coherence tomography -- Cancer diagnosis; Lasers in engineering -- Periodicals; Optical measurements -- Periodicals; Optics -- Periodicals; Lasers en ingénierie -- Périodiques; Mesures optiques -- Périodiques; Optique -- Périodiques; 621.36605
• DOI: 10.1016/j.optlaseng.2020.106395
• Languages: English
• ISSNs: 0143-8166

Abstract

Highlights: Depth-resolved images of normal and abnormal human large intestine are given. The crypt cells and intercellular structures of normal intestine were observed. Birefringence image of infiltration of cancerous intestine were observed. Abstract: A polarization-sensitive full-field optical coherence tomography (PS-FFOCT) system was developed, which is capable of revealing both the reflectivity and phase-retardation caused by the anisotropic samples simultaneously with the lateral resolution of 0.98 µm and the axial resolution of 2.4 µm. The system was used to generate depth-resolved en face isotropic intensity images and birefringent images of the normal and abnormal large intestine tissue samples of human in vitro. To our knowledge, for the first time, the intestine gland cells and intercellular structures of normal intestine and infiltration of metaplasia of cancerous intestine tissue were observed in the en face images obtained with PS-FFOCT device. The resolution and effects of measurement errors are discussed. The results obtained in this work demonstrated the potential applications of PS-FFOCT technique in the rapid intra-operation diagnosis of cancers.