NDW, encoding a receptor-like protein kinase, regulates plant growth, cold tolerance and susceptibility to Botrytis cinerea in tomato. (December 2020)

• Record Type: Journal Article
• Authors: Xu, Wei; Gao, Shenghua; Song, Jianwen; Yang, Qihong; Wang, Taotao; Zhang, Yuyang; Zhang, Junhong; Li, Hanxia; Yang, Changxian; Ye, Zhibiao
• Is Part Of: Plant science. Volume 301(2020)
• Publication Date: 2020-12
• Subjects: ABA abscisic acid -- CBF C-repeat-binding factor -- gDNA genomic DNA -- InDel insertion/deletion -- IL introgression line -- MDA malondialdehyde -- ORF open reading frame -- qRT-PCR quantitative real-time PCR -- RLKs receptor-like protein kinases -- GFP green fluorescence protein -- RFP red fluorescence protein; Tomato -- Necrotic dwarf -- Receptor like protein kinase -- Map-based cloning; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2020.110684
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: The phenotype of necrotic dwarf (ndw) mutant introgression line (IL) 6-2 is caused by decreased expression of NDW, which encodes a receptor-like protein kinase. Overexpression of NDW can restore both of the semi-dwarf and necrotic phenotype in IL6-2 at low temperatures. Overexpression of NDW significantly reduces susceptibility to Botrytis cinerea. Knockdown NDW in M82 plants increases the sensitivity to B. cinerea. NDW affects the expression of genes related to the abscisic acid (ABA) signaling pathway. Abstract: Plants utilize different mechanisms to respond and adapt to continuously changing environmental factors. Receptor-like protein kinases (RLKs) comprise one of the largest families of plant transmembrane signaling proteins, which play critical and diverse roles in plant growth, development, and stress response. Here, we identified the necrotic dwarf (ndw) mutant introgression line (IL) 6-2, which demonstrated stunting, leaf curl, and progressive necrosis at low temperatures. Based on map-based cloning and transgenic analysis, we determined that the phenotype of ndw mutant is caused by decreased expression of NDW, which encodes an RLK. NDW is a plasma membrane and cytoplasmic located protein. Overexpression of NDW can restore both of the semi-dwarf and necrotic phenotype in IL6-2 at low temperatures, further we found that NDW could significantly reduce susceptibility to Botrytis cinerea. On the contrary, knockdown NDW in M82 plants could increase the sensitivity to B. cinerea. Furthermore, transcriptional expression analysis showed that NDW affects the expression of genes related to the abscisic acid (ABA) signaling pathway. Taken together, these results indicate that NDW plays an important role in regulating plant growth, cold tolerance and mitigating susceptibility to Botrytis cinerea.