The effect of β-cyclocitral treatment on the carotenoid content of transgenic Marsh grapefruit (Citrus paradisi Macf.) suspension-cultured cells. (December 2020)

• Record Type: Journal Article
• Authors: Zheng, Xiongjie; Zhu, Kaijie; Ye, Junli; Price, Elliott J.; Deng, Xiuxin; Fraser, Paul D.
• Is Part Of: Phytochemistry. Volume 180(2020)
• Publication Date: 2020-12
• Subjects: Citrus paradis -- Rutaceae -- Citrus -- Suspension-cultured cells -- Chemical elicitor -- β-yclocitral -- Carotenoid -- Provitamin A; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2020.112509
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: This work reports the development of suspension culture system of transgenic Marsh grapefruit ( Citrus paradisi Macf., Rutaceae) callus overexpressing bacterial phytoene synthase; and the use of this suspension culture to investigate the effects of β-cyclocitral on carotenoid content and composition. At a β-cyclocitral concentration of 0.5 mM and after ten days cultivation, analysis of the carotenoids showed a significant increase in the content of β-, α-carotene, and phytoene predominantly. The maximal increase in total provitamin A carotenoids content following β-cyclocitral application was ~2-fold higher than the control, reaching 245.8 μg/g DW. The trend for increased transcript levels of biosynthetic genes PSY and ZDS correlated with the enhancement of the content of these carotenes following β-cyclocitral treatment and GC-MS based metabolite profiling showed significant changes of metabolite levels across intermediary metabolism. These findings suggest that β-cyclocitral can act as a chemical elicitor, to enhance the formation of carotenes in citrus suspension-cultured cells (SCC), which could be utilized in studying the regulation of carotenoid biosynthesis and biotechnological application to the renewable production of nutritional carotenoids. Graphical abstract: The suspension cultured cells (SCC) of transgenic Marsh grapefruit callus treated with 0.5 mM β-cc for 12 days have a higher content of α/β-carotene and visually turn redder in coloration compared to the control SCC. Image 1 Highlights: β-cyclocitral application enhanced carotenes content of Citrus paradisi ' suspension-cultured cells (SCC). β-cyclocitral application upregulate the expression of early carotenoid pathway genes in citrus SCC. β-cyclocitral application affect intermediary metabolite pools of citrus SCC.