Perfluorocarbon‐Based 19F MRI Nanoprobes for In Vivo Multicolor Imaging. (27th November 2018)

• Record Type: Journal Article
• Authors: Akazawa, Kazuki; Sugihara, Fuminori; Nakamura, Tatsuya; Matsushita, Hisashi; Mukai, Hiroaki; Akimoto, Rena; Minoshima, Masafumi; Mizukami, Shin; Kikuchi, Kazuya
• Is Part Of: Angewandte Chemie. Volume 130:Number 51(2018)
• Page Start: 16984
• Page End: 16989
• Publication Date: 2018-11-27
• Subjects: Fluor -- Kernspintomographie -- Kontrastmittel -- Nanopartikel -- Vielfarbenbildgebung; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201810363
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: In vivo multicolor imaging is important for monitoring multiple biomolecular or cellular processes in biology. 19 F magnetic resonance imaging (MRI) is an emerging in vivo imaging technique because it can non‐invasively visualize 19 F nuclei without endogenous background signals. Therefore, 19 F MRI probes capable of multicolor imaging are in high demand. Herein, we report five types of perfluorocarbon‐encapsulated silica nanoparticles that show 19 F NMR peaks with different chemical shifts. Three of the nanoprobes, which show spectrally distinct 19 F NMR peaks with sufficient sensitivity, were selected for in vivo multicolor 19 F MRI. The nanoprobes exhibited 19 F MRI signals with three colors in a living mouse. Our in vivo multicolor system could be utilized for evaluating the effect of surface functional groups on the hepatic uptake in a mouse. This novel multicolor imaging technology will be a practical tool for elucidating in vivo biomolecular networks by 19 F MRI. Abstract : Farbenfrohe Kernspintomogramme : Nanopartikel aus Perfluorkohlenwasserstoffen in SiO2 ‐Schalen – PFCE@SiO2, TPFBME@SiO2 und PFTBA@SiO2 – ergeben 19 F‐NMR‐Signale mit unterschiedlichen chemischen Verschiebungen. Diese Nanosonden ermöglichen die Multiplex‐ 19 F‐MRI‐Analyse von Phantomen sowie die Dreifarben‐ 19 F‐Bildgebung in einer lebenden Maus.