Design of spontaneously blinking fluorophores for live-cell super-resolution imaging based on quantum-chemical calculations. Issue 86 (6th October 2020)

• Record Type: Journal Article
• Authors: Tachibana, Ryo; Kamiya, Mako; Morozumi, Akihiko; Miyazaki, Yoshiyuki; Fujioka, Hiroyoshi; Nanjo, Aika; Kojima, Ryosuke; Komatsu, Toru; Ueno, Tasuku; Hanaoka, Kenjiro; Yoshihara, Toshitada; Tobita, Seiji; Urano, Yasuteru
• Is Part Of: Chemical communications. Volume 56:Issue 86(2020)
• Page Start: 13173
• Page End: 13176
• Publication Date: 2020-10-06
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc05126h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Spontaneously blinking fluorophores are powerful tools for live-cell super-resolution imaging under physiological conditions. Abstract : Spontaneously blinking fluorophores are powerful tools for live-cell super-resolution imaging under physiological conditions. Here we show that quantum-chemical calculations can predict key parameters for fluorophore design. We applied this methodology to develop a spontaneously blinking fluorophore with yellow fluorescence for super-resolution imaging of microtubules in living cells.