Screening and identification of biomarkers associated with the diagnosis and prognosis of lung adenocarcinoma. Issue 10 (16th July 2020)

• Record Type: Journal Article
• Authors: Wang, Yanyun; Zhang, Lin; Chen, Yitong; Li, Man; Ha, Minwen; Li, Songbai
• Is Part Of: Journal of clinical laboratory analysis. Volume 34:Issue 10(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-07-16
• Subjects: bioinformatics analysis -- biomarker -- lung adenocarcinoma -- prognosis -- single nucleotide polymorphism; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.23450
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: In this study, we aimed to identify the pathogenesis and prognostic biomarkers of lung adenocarcinoma (LUAD). Methods: Differentially expressed mRNAs (DEmRNAs) and single nucleotide polymorphism (SNP) mutant genes were screened. In addition, enrichment and protein‐protein interaction (PPI) network analyses of the SNP‐mutated genes were performed. Thereafter, the correlation between gene mutation and expression was analyzed. Finally, the mutated genes associated with LUAD prognosis were validated on the basis of The Cancer Genome Atlas (TCGA) database. Results: A total of 2502 DEmRNAs were initially screened in this study. We identified 756 SNP‐mutated genes from more than 30 cases. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that the mutated genes involved in LUAD were mainly associated with the ECM‐receptor interaction, focal adhesion, and calcium signaling pathways. Tumor protein p53 ( TP53 ) and neurexin 1 ( NRXN1 ) with the higher degree were chosen as the hub genes in the PPI network. In addition, the correlation analysis revealed six genes, including assembly factor for spindle microtubules ( ASPM ), centromere protein F ( CENPF ), contactin 3 ( CNTN3 ), catenin delta 2 ( CTNND2 ), PKHD1 like 1 ( PKHD1L1 ), and semaphorin 6D ( SEMA6D ), and three SNP mutations at ASPM rs368020495, CENPF rs762653487, and PKHD1L1 rs768349010 sites that were found to be associated with LUAD prognosis. Further validation showed that among the aforementioned six mutated genes, CENPF was upregulated and SEMA6D was downregulated. Conclusion: CENPF, SEMA6D, TP53, and NRXN1 were found to be closely associated with the development of LUAD. Abstract : CENPF, SEMA6D, TP53, and NRXN1 were found to be closely associated with the development of LUAD.