Dapsone hydroxylamine-mediated alterations in human red blood cells from endometriotic patients. (2nd December 2017)

• Record Type: Journal Article
• Authors: Andrisani, Alessandra; Donà, Gabriella; Sabbadin, Chiara; Dall'Acqua, Stefano; Tibaldi, Elena; Roveri, Antonella; Bosello Travain, Valentina; Brunati, Anna Maria; Ambrosini, Guido; Ragazzi, Eugenio; Armanini, Decio; Bordin, Luciana
• Is Part Of: Gynecological endocrinology. Volume 33:Number 12(2017)
• Page Start: 928
• Page End: 932
• Publication Date: 2017-12-02
• Subjects: Endometriosis -- dapsone -- DDS-NHOH -- red blood cells -- oxidative stress; Endocrine gynecology -- Periodicals; Generative organs, Female -- Diseases -- Periodicals; 618.1
• DOI: 10.1080/09513590.2017.1332177
• Languages: English
• ISSNs: 0951-3590

Abstract

Abstract: Endometriosis, an estrogen-dependent chronic gynecological disease in women of reproductive age, is characterized by a systemic inflammation status involving also red blood cells (RBCs). In this study, we evaluated how the protein oxidative status could be involved in the worsening of RBC conditions due to dapsone intake in endometriotic women in potential treatment for skin or infection diseases. Blood samples from two groups of volunteers, control group (CG) and endometriosis patient group (PG), were analyzed for their content of band 3 tyrosine phosphorylation (Tyr-P) and high molecular weight aggregate (HMWA) in membranes, and glutathione (GSH) content and carbonic anhydrase (CA) activity in cytosol. In endometriotic patients, RBC showed the highest level of oxidative-related alterations both in membrane and cytosol. More interestingly, the addition of dapsone hydroxylamine (DDS-NHOH) could induce further increase of both membranes and cytosol markers, with an enhancement of CA activity reaching about 66% of the total cell enzyme amount. In conclusion, in PG the systemic inflammatory status leads to the inability of counteracting adjunctive oxidative stress, with a potential involvement of CA-related pathologies, such as glaucoma. Hence, the importance of the evaluation of therapeutic approaches worsening oxidative imbalance present in PG RBC is underlined.