Non‐targeted metabolomics approach to assess the brown marine macroalga Dictyota dichotoma as a functional food using liquid chromatography with mass spectrometry. Issue 5 (19th February 2020)

• Record Type: Journal Article
• Authors: Dixit, Dhara; Reddy, C.R.K.; Trivedi, M.H.; Gadhavi, Devesh K.
• Is Part Of: Separation science plus. Volume 3:Issue 5(2020)
• Page Start: 140
• Page End: 149
• Publication Date: 2020-02-19
• Subjects: Dictyota dichotoma -- functional foods -- Kachchh -- liquid chromatography -- non‐targeted metabolomics; Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1002/sscp.201900109
• Languages: English
• ISSNs: 2573-1815

Abstract

Abstract: The study elucidates presence of 16 potential putative metabolites in Dictyota dichotoma through non‐targeted secondary metabolite profile analysis using electrospray ionization negative mode of liquid chromatography with time‐of‐flight mass spectrometry. Two fatty acyl glycosides viz; 5‐megastigmen‐7‐yne‐3, 9‐diol 9‐glucoside (biomarker for food consumption) and (3S, 7E, 9S)‐9‐hydroxy‐4, 7‐megastigmadien‐3‐one 9‐glucoside (nutrient, emulsifier, surfactant), an o‐glycosyl compound 5‐megastigmen‐7‐yne‐3, 9‐diol 3‐glucoside (biomarker for food consumption), all with m/z value 370.19 were detected. An acyclic monoterpenoid and diterpenoid viz; isodigeranyl and digeranyl (both with m/z 274.26; food biomarkers) were detected. Additionally, (3′x, 5′a, 9′x, 10′b)‐O‐(3‐hydroxy‐6‐oxo‐7‐drimen‐11‐yl) umbelliferone (m/z 396.19; nutrient, emulsifier, surfactant) and kamolonol (m/z 398.2; antibacterial, anti‐tumor) were observed. Two angular pyranocoumarins: mammea C/AB cyclo D (m/z 398.2; food consumption biomarker) and 5‐hydroxy‐8, 8‐dimethyl‐6‐(3‐methyl‐1‐oxobutyl)‐4‐pentyl‐2H, 8H‐benzo[1, 2‐b:3, 4‐b']dipyran‐2‐one (m/z 398.2; fruit constituent) were present. Two terpene lactones ‐ galbanic acid (m/z 398.2; antimicrobial, anticoagulant, antiviral, anti‐inflammatory, anti‐cancer) and asacoumarin A (m/z 398.2; antiprotozoal, antiglycation activity) were detected. Armillaribin (m/z 398.2; food biomarker) along with glycinoeclepin B (m/z 558.28; nutrient, emulsifier, surfactant) was noted. Two flavonoids; (S)‐(E)‐2′‐(3, 6‐dimethyl‐2‐heptenyl)‐3′, 4′, 7‐trihydroxyflavanone and (S)‐(E)‐8‐(3, 6‐dimethyl‐2‐heptenyl)‐4′, 5, 7‐trihydroxyflavanone (m/z 396.19) and a drug triclofos (m/z 227.89; sedative) were detected. This study highlights the metabolic makeup of D. dichotoma collected from Kachchh coast thereby, accentuating the probability of its inclusion as a promising ingredient in functional foods from algal origin.