Bioconversion of organosolv‐treated bamboo into biobutanol integrated with on‐site hydrolytic enzyme production. (25th November 2019)

• Record Type: Journal Article
• Authors: Xu, Ning; He, Aiyong; Xu, Jiaxing; Xin, Fengxue
• Is Part Of: Biofuels, bioproducts and biorefining. Volume 14:Number 2(2020)
• Page Start: 117
• Page End: 126
• Publication Date: 2019-11-25
• Subjects: bamboo -- organosolv pretreatment -- solid state fermentation -- in situ removal -- biobutanol; Biomass energy -- Periodicals; Biological products -- Periodicals; Fuel -- Refining -- Periodicals; 662.8805
• DOI: 10.1002/bbb.2065
• Languages: English
• ISSNs: 1932-104X

Abstract

Abstract: The aim of this study is to convert organosolv‐treated bamboo into biobutanol hydrolyzed by lignocellulosic enzyme mixtures produced on site through solid state fermentation (SSF). Higher xylanase and β ‐xylosidase activity in crude hydrolytic enzyme mixtures produced from untreated bamboo under SSF contributed to a higher saccharification yield (76.1%) than commercial ones (67.3%). The modified organosolv pretreatment under mild conditions, without acid catalysts at 35 °C, could also improve the sugar yield from bamboo efficiently by reducing 29.8% of lignin content. Finally, in situ extraction using biodiesel as the extractant could improve the final butanol titer to 28.4 g L −1 with a yield of 0.24 g g −1 from the organosolv treated bamboo enzymatic hydrolysate, thus demonstrating the first investigation of biobutanol production from bamboo. The results suggested that a crude cellulase complex produced under SSF and organosolv pretreatment could efficiently provide bamboo hydrolysate for biobutanol production without commercial cellulase usage. This has potential for large‐scale biobutanol production from bamboo wastes. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd