Streptomyces cadmiisoli sp. nov., a novel actinomycete isolated from cadmium-contaminated soil. Issue 4 (4th February 2019)

• Record Type: Journal Article
• Authors: Li, Kaiqin; Tang, Xinke; Zhao, Jiarong; Guo, Yihui; Tang, Yongjie; Gao, Jian
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 4(2019)
• Page Start: 1024
• Page End: 1029
• Publication Date: 2019-02-04
• Subjects: Streptomtces cadmiisoli sp. nov. -- cadmium-contaminated soil -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003262
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Streptomyces strain, ZFG47 T, isolated from a cadmium-contaminated soil sample, was taxonomically studied in detail. Strain ZFG47 T formed long, flexuous spiral spore chains consisting of elliptoid spores with spiny surfaces. The cell-wall hydrolysates contained ll -diaminopimelic acid as the diagnostic diamino acid. The major menaquinones consisted of MK-9(H2), MK-9(H4) and MK-9(H8). The major polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were iso-C16 : 0, C16 : 0 and anteiso-C15 : 0 . The 16S rRNA gene sequence-based phylogenetic analysis indicated that this strain belongs to the genus Streptomyces, showing the highest sequence similarity to Streptomyces koyangensis VK-A60 T (98.7 %). However, the digital DNA–DNA hybridization value, the average nucleotide identity value and the MLSA evolutionary distance between this strain and S. koyangensis VK-A60 T showed that it belonged to a distinct species. Furthermore, the novel isolate could be distinctly differentiated from S. koyangensis VK-A60 T by morphological, physiological and biochemical characteristics. On the basis of the evidence from this polyphasic study, it is concluded that strain ZFG47 T represents a novel species of the genus Streptomyces, for which the name Streptomyces cadmiisoli sp. nov. is proposed, with strain ZFG47 T (CICC 11050 T =JCM 32897 T ) as the type strain.