Frateuria defendens sp. nov., bacterium isolated from the yellows grapevine's disease vector Hyalesthes obsoletus. Issue 5 (20th February 2019)

• Record Type: Journal Article
• Authors: Lidor, Ofir; Santos-Garcia, Diego; Mozes-Daube, Netta; Naor, Vered; Cohen, Eyal; Iasur-Kruh, Lilach; Bahar, Ofir; Zchori-Fein, Einat
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 5(2019)
• Page Start: 1281
• Page End: 1287
• Publication Date: 2019-02-20
• Subjects: biological agent -- DLB -- phloem bacteria -- plant pathogens; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003305
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Dyella -like bacterium was previously isolated from the planthopper Hyalesthes obsoletus (Hemiptera). Based on its 16S rRNA gene sequence, strain DHo T was assigned to the family Rhodanobacteraceae with Dyella and Frateuria as its closest relatives. The closest 16S rRNA gene sequences were Frateuria aurantia DSM 6220 T (98.2 %), Dyella thiooxydans ATSB10 T (98 %), Dyella terrae JS14-6 T (97.8 %) and Dyella marensis CS5-B2 T (97.8 %). Strain DHo T is a Gram-negative, aerobic, motile, yellow-pigmented, rod-shaped bacterium. Strain DHo T cells grew well at 28–30 °C and at pH 6.5–7.5 on a nutrient agar plate. DNA–DNA hybridization showed that the relatedness between strain DHo T and D. jiangningensis strain SBZ3-12 T, and F. aurantia DSM 6220 T was 42.7 and 42.6 %, respectively. Ubiquinone Q-8 was the predominant respiratory quinone, and the major fatty acids (>10 %) were iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 . In silico analysis based on phylogenetics and sequence identity at the nucleotide and protein levels suggests that Frateuria is the closest known relative of strain DHo T . Based on the phenotypic, chemotaxonomic and phylogenetic data, strain DHo T was designated as a novel species of the genus Frateuria, for which the name Frateuria defendens sp. nov. is proposed. The type strain is DHo T (=NCCB 100648 T ; =DLB T =DSM 106169 T ).