Pseudomonas asiatica sp. nov., isolated from hospitalized patients in Japan and Myanmar. Issue 5 (27th February 2019)

• Record Type: Journal Article
• Authors: Tohya, Mari; Watanabe, Shin; Teramoto, Kanae; Uechi, Kohei; Tada, Tatsuya; Kuwahara-Arai, Kyoko; Kinjo, Takeshi; Maeda, Shiro; Nakasone, Isamu; Zaw, Ni Ni; Mya, San; Zan, Khin Nyein; Tin, Htay Htay; Fujita, Jiro; Kirikae, Teruo
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 69:Issue 5(2019)
• Page Start: 1361
• Page End: 1368
• Publication Date: 2019-02-27
• Subjects: Pseudomonas -- human pathogen; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.003316
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A novel Gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, RYU5 T, was isolated from a stool sample of an inpatient at a hospital in Okinawa, Japan. The optimal growth temperature of RYU5 T was 30 °C. Phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA, rpoB, rpoD and gyrB gene s, showed that RYU5 T was a member of the Pseudomonas putida group and was located close to Pseudomonas monteilii and P. putida . Whole-genome comparisons, using average nucleotide identity and digital DNA–DNA hybridization, confirmed that strain RYU5 T should be classified as a novel species of Pseudomonas . Phenotypic characterization tests showed that utilization of d -mannose, d -serine, l -arabinose and d -fructose could distinguish this strain from other related species of the genus Pseudomonas . Based on genetic and phenotypic evidence, strain RYU5 T should be classified as a novel species, for which the name Pseudomonas asiatica sp. nov. is proposed. The type strain is RYU5 T (=DSM 107182 T, =JCM 32716 T ), with a DNA G+C content of 62.25 mol%.