PH‐Dependent Chloride Transport by Pseudopeptidic Cages for the Selective Killing of Cancer Cells in Acidic Microenvironments. (1st August 2019)

• Record Type: Journal Article
• Authors: Tapia, Lucía; Pérez, Yolanda; Bolte, Michael; Casas, Josefina; Solà, Jordi; Quesada, Roberto; Alfonso, Ignacio
• Is Part Of: Angewandte Chemie. Volume 131:Number 36(2019)
• Page Start: 12595
• Page End: 12598
• Publication Date: 2019-08-01
• Subjects: Anionophore -- Krebs -- NMR-Spektroskopie -- Supramolekulare Chemie -- Wirt-Gast-Systeme; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201905965
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Acidic microenvironments in solid tumors are a hallmark of cancer. Inspired by that, we designed a family of pseudopeptidic cage‐like anionophores displaying pH‐dependent activity. When protonated, they efficiently bind chloride anions. They also transport chloride through lipid bilayers, with their anionophoric properties improving at acidic pH, suggesting an H + /Cl − symport mechanism. NMR studies in DPC micelles demonstrate that the cages bind chloride within the lipid phase. The chloride affinity and the chloride‐exchange rate with the aqueous bulk solution are improved when the pH is lowered. This increases cytotoxicity towards lung adenocarcinoma cells at the pH of the microenvironment of a solid tumor. These properties depend on the nature of the amino‐acid side chains of the cages, which modulate their lipophilicity and interactions with the cell membrane. This paves the way towards using pH as a parameter to control the selectivity of cytotoxic ionophores as anticancer drugs. Abstract : Sauer macht giftig : Kleine pseudopeptidische Käfige zeigen verbesserte Bindung und Transport von Chlorid über eine Lipidmembran hinweg bei sinkendem pH‐Wert der Umgebung. Dies erhöht ihre Zytotoxizität für Lungenadenokarzinomzellen in Umgebungen, die jene im Umfeld solider Tumoren imitieren.