Cell disruption pre-treatments towards an effective recovery of oil from Yarrowia lipolytica oleaginous yeast. (September 2019)

• Record Type: Journal Article
• Authors: Drévillon, Lucie; Koubaa, Mohamed; Nicaud, Jean-Marc; Vorobiev, Eugène
• Is Part Of: Biomass and bioenergy. Volume 128(2019)
• Publication Date: 2019-09
• Subjects: Yarrowia lipolytica -- Oil recovery -- High-pressure homogenization -- Ultrasound -- Pulsed electric fields -- High voltage electrical discharges; CFA cyclopropane fatty acid -- DM dry matter -- GC-FID gas chromatography-flame ionization detector -- HPEF high pulsed electric fields -- HPH high-pressure homogenization -- HVED high voltage electrical discharges -- ME mechanical expression -- MPEF-ME moderate pulsed electric fields assisted mechanical expression -- SCO single cell oil -- US ultrasound; Biomass energy -- Periodicals; Biomass -- Periodicals; Energy-Generating Resources -- Periodicals; Bioénergie -- Périodiques; 333.9539
• DOI: 10.1016/j.biombioe.2019.105320
• Languages: English
• ISSNs: 0961-9534

Abstract

Abstract: The aim of this work was to evaluate the efficiency of different cell disruption techniques on oil extraction from Y. lipolytica oleaginous yeast. The techniques tested were mechanical expression (ME), moderate pulsed electric field assisted mechanical expression (MPEF-ME), high pulsed electric fields (HPEF), high voltage electrical discharges (HVED), ultrasound (US), and high-pressure homogenization (HPH). The impact of each cell disruption technique on fatty acid composition was investigated. Results show that the most efficient technique for Y. lipolytica disruption was HPH leading to the obtaining of 83.8 ± 4.8% oil extraction yield, compared to only 19.8 ± 0.5% for the control, without any pre-treatment. In terms of fatty acid composition, HVED induced significant changes in almost all the analyzed fatty acids, while US was the softest technique. HPH, ME, and MPEF-ME induced changes in the content of some fatty acids. Highlights: Efficiency of cell disruption technologies for oil recovery from Yarrowia lipolytica yeast. High-pressure homogenization (HPH) was the most efficient technique among all tested. A yield of 83.85 ± 4.77% was obtained for HPH, compared to only 19.79 ± 0.48% for the control. Although the most efficient, HPH was the most energy-consuming technique with 134 kJ/g of oil.