(Poly)phenol metabolites from Arbutus unedo leaves protect yeast from oxidative injury by activation of antioxidant and protein clearance pathways. (May 2017)

• Record Type: Journal Article
• Authors: Jardim, Carolina; Macedo, Diana; Figueira, Inês; Dobson, Gary; McDougall, Gordon J.; Stewart, Derek; Ferreira, Ricardo B.; Menezes, Regina; Santos, Cláudia N.
• Is Part Of: Journal of functional foods. Volume 32(2017)
• Page Start: 333
• Page End: 346
• Publication Date: 2017-05
• Subjects: PDM (Poly)phenol Digested Metabolites -- ROS reactive oxygen species -- GAE gallic acid equivalents -- ORAC Oxygen Radical Absorbance Capacity -- LC-MS Liquid Chromatography Mass Spectrometry -- CSM complete supplement mixture -- R.F.U. relative fluorescence units -- H2DCFDA 2′, 7′-dichlorofluorescein diacetate -- DCF 2′, 7′-dichlorofluorescein -- OD optical density -- DHE Dihydroethidium -- MFI mean fluorescence intensity -- MIQE Minimum Information for publication of Quantitative real-time PCR Experiments -- GSH reduced glutathione -- GSSG oxidised glutathione -- αSyn alpha-synuclein; Polyphenols -- Bioaccessibility -- Oxidative stress -- Saccharomyces cerevisiae -- Yap1 -- Redox homoeostasis; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2017.03.010
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: A. unedo leaf phenolics protects S. cerevisiae from oxidative stress. A. unedo leaf phenolics activates cellular antioxidant machinery. A. unedo leaf phenolics modulates SOD1, SOD2, RPN4, GSH1 and GSH2 in cells exposed to H2 O2 . A. unedo constitutes a powerful matrix to be explored as a functional food. A. unedo nutraceutical potential is relevant to oxidative stress-associated diseases. Abstract: Oxidative stress is a key process in several diseases and a growing body of preclinical/clinical evidence has pushed forward the health benefits of (poly)phenols. The protective potential of Corema album and Arbutus unedo (Poly)phenol Digested Metabolites (PDM), generated after in vitro digestion was evaluated. Chemical alterations caused by the digestion led to alterations of compounds reactivity. A. unedo leaf PDM arose as the fraction conferring the highest protective activity to the wild type and oxidative stress-sensitive yap1 yeast strains challenged with H2 O2 . Our mechanistic studies disclosed the effect of A. unedo leaf PDM on cell redox homeostasis and mitochondrial function, and identified HAP4, SOD1, SOD2, GSH1 and GLR1 as mediators of cellular protection. Also, RPN4 - and ATG8 -associated protein clearance pathways were revealed as putative targets. Overall, these data suggest the nutraceutical potential of A. unedo leaf PDM as a functional food for human health.