The use of germicidal ultraviolet light, vaporized hydrogen peroxide and dry heat to decontaminate face masks and filtering respirators contaminated with a SARS-CoV-2 surrogate virus. Issue 3 (November 2020)

• Record Type: Journal Article
• Authors: Ludwig-Begall, L.F.; Wielick, C.; Dams, L.; Nauwynck, H.; Demeuldre, P-F.; Napp, A.; Laperre, J.; Haubruge, E.; Thiry, E.
• Is Part Of: Journal of hospital infection. Volume 106:Issue 3(2020)
• Page Start: 577
• Page End: 584
• Publication Date: 2020-11
• Subjects: SARS-CoV-2 -- PRCV -- Decontamination (UV, H2O2, dry heat) -- Respirator -- Surgical mask; Cross infection -- Periodicals; Cross infection -- Prevention -- Periodicals; Nosocomial infections -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Cross Infection -- Periodicals; Cross Infection -- prevention & control -- Periodicals; Infection Control -- Periodicals; Electronic journals; 614.44
• DOI: 10.1016/j.jhin.2020.08.025
• Languages: English
• ISSNs: 0195-6701

Abstract

Summary: Background: In the context of the ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, the supply of personal protective equipment remains under severe strain. To address this issue, re-use of surgical face masks and filtering facepiece respirators has been recommended; prior decontamination is paramount to their re-use. Aim: We aim to provide information on the effects of three decontamination procedures on porcine respiratory coronavirus (PRCV)-contaminated masks and respirators, presenting a stable model for infectious coronavirus decontamination of these typically single-use-only products. Methods: Surgical masks and filtering facepiece respirator coupons and straps were inoculated with infectious PRCV and submitted to three decontamination treatments, ultraviolet (UV) irradiation, vaporized H2 O2, and dry heat treatment. Viruses were recovered from sample materials and viral titres were measured in swine testicle cells. Findings: UV irradiation, vaporized H2 O2 and dry heat reduced infectious PRCV by more than three orders of magnitude on mask and respirator coupons and rendered it undetectable in all decontamination assays. Conclusion: This is the first description of stable disinfection of face masks and filtering facepiece respirators contaminated with an infectious SARS-CoV-2 surrogate using UV irradiation, vaporized H2 O2 and dry heat treatment. The three methods permit demonstration of a loss of infectivity by more than three orders of magnitude of an infectious coronavirus in line with the United States Food and Drug Administration policy regarding face masks and respirators. It presents advantages of uncomplicated manipulation and utilization in a BSL2 facility, therefore being easily adaptable to other respirator and mask types.