Fabrication and Characterization of a Novel Berberine-oleanolic Acid Delivery Collagen I scaffold. (September 2020)

• Record Type: Journal Article
• Authors: Chen, Zongming; Li, Yongsheng; Dai, Yao; Zhou, Zheng; Hu, Yibing; Liu, Hairong
• Is Part Of: Journal of physics. Volume 1637(2020)
• Publication Date: 2020-09
• Subjects: Physics -- Congresses; 530.5
• DOI: 10.1088/1742-6596/1637/1/012105
• Languages: English
• ISSNs: 1742-6588

Abstract

Abstract: Postoperative bacterial infection greatly threatens prognosis of patients following tissue engineering implantation and surgical operation. In this study, a novel antibacterial tissue engineering porous scaffold has been prepared, by blending a newly synthesized berberine-oleanolic acid (HL-9) with collagen (COL I) via freeze-drying. It showed that the HL-9 delivery COL I scaffolds displayed a uniform porous three-dimensional structure, high porosity and good water absorption. These HL-9 delivery COL I porous scaffolds (HL-9/COL I scaffolds) are able to release HL-9 steadily for 15 days. HL-9/COL I scaffolds, which contains, 4% and 5% HL-9 respectively, exhibited a similar antibacterial activity against Gram-positive and Gram-negative bacteria. Hence, the HL-9/COL I scaffolds containing 3% HL-9 is used to test the biocompatibility. By employing Alamar Blue assays and FDA staining, the proliferation of MG-63 cultured with 3%HL-9/COL I scaffolds is similar with that cultured with COL I scaffolds. In conclusion, the 3%HL-9/COL I scaffolds showed a good biocompatibility and antibacterial activity, suggesting that it may be applied in tissue engineering in the future.