Airborne fungus-induced biosynthesis of anthocyanins in Arabidopsis thaliana via jasmonic acid and salicylic acid signaling. (November 2020)

• Record Type: Journal Article
• Authors: Liu, Yajun; Li, Ming; Li, Tongtong; Chen, Yujie; Zhang, Lingjie; Zhao, Guifu; Zhuang, Juhua; Zhao, Wenyan; Gao, Liping; Xia, Tao
• Is Part Of: Plant science. Volume 300(2020)
• Publication Date: 2020-11
• Subjects: JA jasmonic acid -- SA salicylic acid -- CHS chalcone synthase -- DFR dihydroflavonol rеductase -- ANS anthocyanin synthase -- FLS flavonol synthase -- MYB myeloblastosis -- bHLH basichelix–loop–helix -- WD40 tryptophan-aspartic acid repeat protein -- TTG1 TRANSPARENT TESTAGLABRA1 -- GL3 Glabra3 -- TT8 Transparent Testa8 -- MBW MYB–bHLH–WD40 complex -- DFR NADPH-dependent dihydroflavonol reductase -- ANS/LDOX anthocyanidin synthase/leucoanthocyanidin dioxygenase -- UFGT UDP-Glc:flavonoid 3-O-glucosyltransferase -- COI1 coronatine insensitive 1 -- JAZ jasmonate ZIM-domain protein -- SCFCOI1 SCF-type E3 ubiquitin ligase -- IBU ibuprofen -- AIP 2-aminoindan-2-phosphonic acid -- UPLC ultra performance liquid chromatography -- QQQ triple quadrupole mass spectrometer -- MS mass spectrometry -- PLA2 Phosholipase A2 -- LOX2 lipoxygenase 2 -- LOX4 lipoxygenase 4 -- AOC1 allene oxide cyclase 1 -- AOC2 allene oxide cyclase 2 -- MAMPs microbe-associated molecular patterns -- PAMPs pathogen-associated molecular patterns -- DEGs differentially expressed gene -- FPKM fragments per kilobase per million reads; Anthocyanin biosynthesis -- A. thaliana -- Jasmonic acid -- Salicylic acid -- Fungus -- MYB–bHLH–WD40 complex; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2020.110635
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: The fungus Penicillium corylophilum, triggered jasmonic acid, salicylic acid, and anthocyanin accumulation in A. thaliana . Both JA and SA signaling in the regulation of fungus-induced anthocyanin biosynthesis in A. thaliana . Multiple MYB–bHLH–WD40 complexes in the fungus P. Corylophilum induced anthocyanin accumulation in A. thaliana . Abstract: Anthocyanins are plant-specific pigments, the biosynthesis of which is stimulated by pathogen infection in several plant species. A. thaliana seedlings injected with airborne fungi can accumulate a high content of anthocyanins. The mechanism involved in fungus-induced anthocyanin accumulation in plants has not been fully described. In this study, the fungus Penicillium corylophilum (P. corylophilum), isolated from an Arabidopsis culture chamber, triggered jasmonic acid (JA), salicylic acid (SA), and anthocyanin accumulation in A. thaliana . Inhibitors of JA and SA biosynthesis suppressed the anthocyanin accumulation induced by P. corylophilum . The anthocyanin content was minimal in both the null mutant of JA-receptor coi1 and the null mutant of SA-receptor npr1 under P. corylophilum stimulation. The results indicate that JA and SA signaling mediated fungus-induced anthocyanin biosynthesis in A. thaliana . P. corylophilum led to different levels of anthocyanin generation in null mutants for MYB75, bHLH, EGL3, and GL3 transcription factors and WD40 protein, demonstrating that multiple MYB–bHLH–WD40 transcription factor complexes participated in fungus-induced anthocyanin accumulation in A. thaliana. The present study will help further elucidate the mechanism of plant resistance to pathogen infection.