Expression of DCP1a in gastric cancer and its biological function and mechanism in chemotherapy resistance in gastric cancer cells. Issue 11 (November 2020)

• Record Type: Journal Article
• Authors: Ruan, Tuo; Zhang, Yazhi; Liu, Weizhen; Li, Yuan; Wang, Dianshi; Du, Zhouyuan; Tao, Kaixiong; Wu, Chuanqing
• Is Part Of: Digestive and liver disease. Volume 52:Issue 11(2020)
• Page Start: 1351
• Page End: 1358
• Publication Date: 2020-11
• Subjects: Gastric cancer -- DCP1a -- Drug resistance -- Proliferation -- Invasion and metastasis; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; 616.33005
• DOI: 10.1016/j.dld.2020.06.031
• Languages: English
• ISSNs: 1590-8658

Abstract

Abstract: Aims: To detect the role of DCP1a in gastric cancer. To estimate the effect of DCP1a in gastric cancer cells on proliferation, invasion, migration and anti-drug behavior in vitro by down-regulating its expression. Methods: Using IHC staining and Western blot to check the expression of DCP1a in tissues and the cell lines. SGC7901 and BGC823 cells were transfected with DCP1a siRNA, and the expression of DCP1a protein and mRNA were detected. The cell proliferation rate was detected by MTT assay and plate cloning assay. Transwell assay was used to detect the change of cell metastasis. The inhibition rates of cells to chemotherapy were detected by MTT assay. And signal pathways were also detected. Results: The expression of DCP1a in cancer tissues is higher ( p < 0.05), and higher expression of DCP1a is related to poor prognosis. After down-regulating the expression of DCP1a in cells, the proliferation rates, migration abilities and chemotherapy resistance decrease. We find that the expression of MRP-1 and the activation of AKT and STAT3 pathways might be involved in regulation. Conclusion: The high expression of DCP1a might be associated with cancer development and prognosis. Down-regulating the expression of DCP1a will help to reduce chemotherapy resistance, which will help with further improvement of chemotherapy in gastric cancer.