Protein‐Controlled Actuation of Dynamic Nucleic Acid Networks by Using Synthetic DNA Translators. Issue 46 (2nd September 2020)

• Record Type: Journal Article
• Authors: Bertucci, Alessandro; Porchetta, Alessandro; Del Grosso, Erica; Patiño, Tania; Idili, Andrea; Ricci, Francesco
• Is Part Of: Angewandte Chemie international edition. Volume 59:Issue 46(2020)
• Page Start: 20577
• Page End: 20581
• Publication Date: 2020-09-02
• Subjects: aptamers -- DNA strand displacement -- DNA-binding proteins -- dynamic DNA nanotechnology -- synthetic biology; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.202008553
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Integrating dynamic DNA nanotechnology with protein‐controlled actuation will expand our ability to process molecular information. We have developed a strategy to actuate strand displacement reactions using DNA‐binding proteins by engineering synthetic DNA translators that convert specific protein‐binding events into trigger inputs through a programmed conformational change. We have constructed synthetic DNA networks responsive to two different DNA‐binding proteins, TATA‐binding protein and Myc‐Max, and demonstrated multi‐input activation of strand displacement reactions. We achieved protein‐controlled regulation of a synthetic RNA and of an enzyme through artificial DNA‐based communication, showing the potential of our molecular system in performing further programmable tasks. Abstract : Using proteins to actuate DNA systems is challenging because it requires non‐trivial binding‐induced mechanisms. We demonstrate a strategy to perform DNA‐based molecular operations controlled by DNA‐binding proteins using synthetic DNA translators that convert specific protein‐binding events into trigger inputs through a programmable structural transition, allowing the establishment of artificial biomolecular communication pathways.