Multi‐crystal data collection using synchrotron radiation as exemplified with low‐symmetry crystals of Dps. Issue 11 (2nd November 2020)

• Record Type: Journal Article
• Authors: Kovalenko, Vladislav; Popov, Alexander; Santoni, Gianluca; Loiko, Natalia; Tereshkina, Ksenia; Tereshkin, Eduard; Krupyanskii, Yurii
• Is Part Of: Acta crystallographica. Volume 76:Issue 11(2020:Nov.)
• Page Start: 568
• Page End: 576
• Publication Date: 2020-11-02
• Subjects: protein crystallography -- muti‐crystal data collection -- clustering analysis; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X20012571
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : The successful structure determination of a triclinic form of Dps from Escherichia coli using multi‐crystal data‐collection techniques is described. Abstract : Multi‐crystal data collection using synchrotron radiation was successfully applied to determine the three‐dimensional structure of a triclinic crystal form of Dps from Escherichia coli at 2.0 Å resolution. The final data set was obtained by combining 261 partial diffraction data sets measured from crystals with an average size of approximately 5 µm. The most important features of diffraction data measurement and processing for low‐symmetry crystals are discussed.