Behind the curtain of the compartmentalization process: Exploring how xylem vessel diameter impacts vascular pathogen resistance. (24th September 2020)

• Record Type: Journal Article
• Authors: Pouzoulet, Jérôme; Rolshausen, Philippe E.; Charbois, Rémi; Chen, Jinliang; Guillaumie, Sabine; Ollat, Nathalie; Gambetta, Gregory A.; Delmas, Chloé E. L.
• Is Part Of: Plant, cell and environment. Volume 43:Number 11(2020)
• Page Start: 2782
• Page End: 2796
• Publication Date: 2020-09-24
• Subjects: grapevine -- mechanistic model -- plant -- tolerance, xylem anatomy; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13848
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: A key determinant of plant resistance to vascular infections lies in the ability of the host to successfully compartmentalize invaders at the xylem level. Growing evidence supports that the structural properties of the vascular system impact host vulnerability towards vascular pathogens. The aim of this study was to provide further insight into the impact of xylem vessel diameter on compartmentalization efficiency and thus vascular pathogen movement, using the interaction between Vitis and Phaeomoniella chlamydospora as a model system. We showed experimentally that an increased number of xylem vessels above 100 μm of diameter resulted in a higher mean infection level of host tissue. This benchmark was validated within and across Vitis genotypes. Although the ability of genotypes to restore vascular cambium integrity upon infection was highly variable, this trait did not correlate with their ability to impede pathogen movement at the xylem level. The distribution of infection severity of cuttings across the range of genotype's susceptibility suggests that a risk‐based mechanism is involved. We used this experimental data to calibrate a mechanistic stochastic model of the pathogen spread and we provide evidence that the efficiency of the compartmentalization process within a given xylem vessel is a function of its diameter. Abstract : We demonstrate that xylem vessel diameter impacts the compartmentalization efficiency of the vascular pathogen Phaeomoniella chlamydospora within a single, and across many, Vitis genotypes. Our results highlight that structural traits of the plant vascular system shape the capability of plant hosts to respond to pathogen infection.