Modulation of β‐catenin levels regulates cranial neural crest patterning and dispersal into first pharyngeal arch. Issue 11 (8th June 2020)

• Record Type: Journal Article
• Authors: Javali, Alok; Lakshmanan, Vairavan; Palakodeti, Dasaradhi; Sambasivan, Ramkumar
• Is Part Of: Developmental dynamics. Volume 249:Issue 11(2020)
• Page Start: 1347
• Page End: 1364
• Publication Date: 2020-06-08
• Subjects: cell‐cell adhesion -- directed differentiation -- N‐cadherin -- neural crest‐like cells -- tissue architecture; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.208
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract: Background: Vertebrate cranial neural crest cells (CNCCs) are multipotent, proximal to the source CNCC form the cranial ganglia. Distally, in the pharyngeal arches, they give rise to the craniofacial skeleton and connective tissues. Fate choices are made as CNCC pattern into distinct destination compartments. In spite of this importance, the mechanism patterning CNCC is poorly defined. Results: Here, we report that a novel β‐catenin‐dependent regulation of N‐Cadherin levels may drive CNCC patterning. In mouse embryos, at the first pharyngeal arch axial level, membrane β‐catenin levels correlate with the extent of N‐cadherin‐mediated adhesion and thus suggest the presence of collective and dispersed states of CNCC. Using in vitro human neural crest model and chemical modulators of β‐catenin levels, we show a requirement for down‐modulating β‐catenin for regulating N‐cadherin levels and cell‐cell adhesion. Similarly, in β‐catenin gain‐of‐function mutant mouse embryos, CNCC fail to lower N‐cadherin levels. This indicates a failure to reduce cell‐cell adhesion, which may underlie the failure of mutant CNCC to populate first pharyngeal arch. Conclusion: We suggest that β‐catenin‐mediated regulation of CNCC adhesion, a previously underappreciated mechanism, underlies the patterning of CNCC into fate‐specific compartments. Key Findings: We provide evidence for a potentially novel patterning event in cranial neural crest development. Neural crest invading the pharyngeal arches appear to transition from a collective to a dispersed state. This apparent transition in cell arrangement is dependent on membrane β‐catenin levels.