One‐step nucleic acid amplification CK19 copy number for sentinel node biopsy in breast cancer: Identification of new cutoffs to predict nonsentinel axillary node involvement. Issue 10 (17th July 2020)

• Record Type: Journal Article
• Authors: Cuffolo, Giulio; Gahir‐Atwal, Harvinder K.; Smith, Brendan
• Is Part Of: Breast journal. Volume 26:Issue 10(2020)
• Page Start: 2002
• Page End: 2005
• Publication Date: 2020-07-17
• Subjects: breast cancer -- CK19 -- one‐step nucleic acid amplification -- total tumor load; Breast -- Diseases -- Periodicals; Breast -- Cancer -- Periodicals; 618.19
• DOI: 10.1111/tbj.13977
• Languages: English
• ISSNs: 1075-122X

Abstract

Abstract: We evaluated novel sentinel node values in breast cancer for one‐step nucleic acid amplification (OSNA) to predict further nodal involvement using various methods in clinically node‐negative disease with a positive OSNA result and subsequent axillary node dissection. 239 patients (118 macrometastatic) were assessed revealing cutoffs of total tumor load (TTL) 44 500 copies/µL (AUROC 0.793); average copy number (ACN) 9450 (AUROC 0.790); and highest copy number (HCN) 46, 000. For macrometastatic patients only: TTL 221 400 copies/µL (AUROC 0.685); ACN 64, 000 (AUROC 0.671); HCN 59 500 (AUROC 0.529). Our data favor TTL and represent one of the largest OSNA macrometastatic predictive series.