Antimicrobial peptide-modified silver nanoparticles for enhancing the antibacterial efficacy. Issue 64 (22nd October 2020)

• Record Type: Journal Article
• Authors: Li, Wenxi; Li, Yongchun; Sun, Pengchao; Zhang, Nan; Zhao, Yidan; Qin, Shangshang; Zhao, Yongxing
• Is Part Of: RSC advances. Volume 10:Issue 64(2020)
• Page Start: 38746
• Page End: 38754
• Publication Date: 2020-10-22
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra05640e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Multifunctional peptides (MFP)-coated silver nanoparticles (MFP@AgNPs) enhanced the antibacterial activity of AgNPs against multidrug-resistant A. baumannii (MDB-AB) strains. Abstract : Antibiotic-resistant bacteria are becoming a serious threat to public health worldwide. To address this problem, we have developed multifunctional peptide (MFP)-coated silver nanoparticles (MFP@AgNPs) for antibacterial studies. MFPs, which can physically adsorb to AgNPs via electrostatic interactions are comprised of a matrix metalloproteinase (MMP) cleavable sequence (PVGLIG), an antimicrobial peptide (tachyplesin-1), and a target peptide (PGP-PEG). The resulting MFP@AgNPs were characterized by various technologies, including UV-vis spectrophotometry, zeta potential analyzer, circular dichroism (CD) spectroscopy, attenuated total reflection-Fourier-transform infrared spectroscopy (ATR-FTIR), and transmission electron microscopy (TEM). The MIC and MBC were investigated against both Gram-positive bacteria and Gram-negative bacteria. The antibacterial activity in vivo was evaluated on MDR-AB (multidrug-resistant Acinetobacter baumannii ) infected mice. We found that MFP@AgNPs exhibited antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria. Compared to bare AgNPs, MFP@AgNPs-1 killed MDR-AB faster and more efficiently. SEM images showed that MFP@AgNPs-1 induced cell disruption via cell membrane damage. In vivo studies further confirmed the enhanced antibacterial activity against MDR-AB infections. The developed MFP@AgNPs-1 reduced the cytotoxicity of AgNPs and enhanced the antibacterial activity against MDR-AB in vitro and in vivo, providing a possible solution against multidrug-resistant bacterial infections.