Ionic magnetic core–shell nanoparticles for DNA extraction. Issue 64 (23rd October 2020)

• Record Type: Journal Article
• Authors: Ali, Tammar Hussein; Mandal, Amar Mousa; Heidelberg, Thorsten; Duali Hussen, Rusnah Syahila; Goh, Ean Wai
• Is Part Of: RSC advances. Volume 10:Issue 64(2020)
• Page Start: 38818
• Page End: 38830
• Publication Date: 2020-10-23
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra05933a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The fabrication ionic magnetic core-shell nanoparticles were simple synthesize with a super-ferromagnetic and small particle size properties, which enabled sufficient DNA particle loading with easy isolation based on an external magnetic field. Abstract : Magnetic nanoparticles with specific surface features are interesting materials for biomedical applications. The combination of molecular interactions on small particles with macroscopic cohesion forces offers unique opportunities. This work reports the synthesis of magnetic core–shell nanoparticles with alkylimidazolium coated surface for effective DNA extraction. A magnetic Fe2 O3 core was coated with a silica shell and functionalized with an organic halide. This enabled a surface coating with organic cations to mediate effective molecular interactions with polyanionic DNA. The large surface area of the ∼20 nm small particles with a magnetization of 25 emu g −1 enabled high DNA particle loading of 1/30 m% with easy isolation based on an external magnetic field. Moreover, the coating of the particles stabilized DNA against ultrasound initiated fragmentation.