An improved 4′-aminomethyltrioxsalen-based nucleic acid crosslinker for biotinylation of double-stranded DNA or RNA. Issue 65 (2nd November 2020)

• Record Type: Journal Article
• Authors: Wielenberg, Kevin; Wang, Miao; Yang, Min; Ozer, Abdullah; Lis, John T.; Lin, Hening
• Is Part Of: RSC advances. Volume 10:Issue 65(2020)
• Page Start: 39870
• Page End: 39874
• Publication Date: 2020-11-02
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra07437c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : An improved photo-crosslinking probe for labeling double-stranded DNA or RNA. Abstract : Nucleic acid crosslinkers that covalently join complementary strands of DNA/RNA have applications in both pharmaceuticals and as biochemical probes. Psoralen is a popular crosslinking moiety that reacts with double stranded DNA and RNA upon exposure to longwave UV light. The commercially available compound EZ-link psoralen-PEG3-biotin has been used in numerous studies to crosslink DNA and double-stranded RNA for genome-wide investigations. Here we present a new probe, AP3B, which uses the psoralen derivative, 4′-aminomethyltrioxsalen, to crosslink and biotinylate nucleic acids. We show that AP3B is 4 to 5 times more effective at labeling DNA in cells and produces a comparable number of crosslinks with over 100 times less compound and less exposure to UV light in vitro than EZ-link psoralen-PEG3-biotin.