Rapid and cytocompatible cell-laden silk hydrogel formation via riboflavin-mediated crosslinking. Issue 41 (1st October 2020)

• Record Type: Journal Article
• Authors: Piluso, Susanna; Flores Gomez, Daniela; Dokter, Inge; Moreira Texeira, Liliana; Li, Yang; Leijten, Jeroen; van Weeren, René; Vermonden, Tina; Karperien, Marcel; Malda, Jos
• Is Part Of: Journal of materials chemistry. Volume 8:Issue 41(2020)
• Page Start: 9566
• Page End: 9575
• Publication Date: 2020-10-01
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d0tb01731k
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : A novel methodology for the development of cell-laden silk hydrogels using a biocompatible photo-initiator. Abstract : Bioactive hydrogels based on naturally-derived polymers are of great interest for regenerative medicine applications. Among naturally-derived polymers, silk fibroin has been extensively explored as a biomaterial for tissue engineering due to its unique mechanical properties. Here, we demonstrate the rapid gelation of cell-laden silk fibroin hydrogels by visible light-induced crosslinking using riboflavin as a photo-initiator, in presence of an electron acceptor. The gelation kinetics were monitored by in situ photo-rheometry. Gelation was achieved in minutes and could be tuned owing to its direct proportionality to the electron acceptor concentration. The concentration of the electron acceptor did not affect the elastic modulus of the hydrogels, which could be altered by varying the polymer content. Further, the biocompatible riboflavin photo-initiator combined with sodium persulfate allowed for the encapsulation of cells within silk fibroin hydrogels. To confirm the cytocompatibility of the silk fibroin formulations, three cell types (articular cartilage-derived progenitor cells, mesenchymal stem cells and dental-pulp-derived stem cells) were encapsulated within the hydrogels, which associated with a viability >80% for all cell types. These results demonstrated that fast gelation of silk fibroin can be achieved by combining it with riboflavin and electron acceptors, which results in a hydrogel that can be used in tissue engineering and cell delivery applications.