Elasticity‐Driven Membrane Budding through Cholesterol Concentration on Supported Lipid Monolayer–Bilayer Junction. Issue 21 (13th September 2020)

• Record Type: Journal Article
• Authors: Lee, Yeon Kyung; Yu, Eui‐Sang; Ahn, Dong June; Ryu, Yong‐Sang
• Is Part Of: Advanced materials interfaces. Volume 7:Issue 21(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-13
• Subjects: cholesterols -- curvatures -- lipid membranes -- membrane budding -- monolayer–bilayer junctions; Materials science -- Periodicals; 620.11
• DOI: 10.1002/admi.202000937
• Languages: English
• ISSNs: 2196-7350

Abstract

Abstract: Membrane budding is an essential process during various biological activities, such as intercellular communication and transportation of life‐sustaining molecules and signals, which primarily occur with the aid of particular proteins. During such processes, the presence of cholesterol and its assembly into particular domains within the membranes have attracted considerable attention due to their unique characteristics in terms of the regulation of signal activities, membrane fluidity, and hormone production during metabolic pathways. However, despite these crucial roles, the precise mechanisms of their spatiotemporal localization toward specific areas and their physiological roles in membrane budding without the assistance of particular proteins remain unclear. Herein, a model membrane platform is reconstituted with lipid monolayer–bilayer junctions, which facilitate the selective concentration of cholesterol on the lipid monolayer regions. In vitro membrane budding is demonstrated by the remaining vesicles where the lipid monolayer membrane is ruptured. The physicochemical approach to the selective concentration of cholesterol and its consequent membrane vesiculation offer important clues to help understand the underlying mechanisms of cholesterol in the lipid‐driven membrane‐budding process. Abstract : Despite its crucial roles in a variety of biological functions, cholesterol‐assisted underlying mechanism on membrane budding remained one of the mysterious biological events. By reconstruction of lipid monolayer–bilayer junctions, cholesterols are selectively concentrated and membrane transformation is monitored from a sheet to vesicles after membrane ruptures. Underlying mechanisms for lipid‐driven membrane bud‐off process are discussed through physicochemical approach.