Selection of a suitable paper membrane for Loop Mediated Isothermal DNA amplification reaction (LAMP) in a point-of-care diagnostic kit – Experimental and CFD analysis. (16th January 2021)

• Record Type: Journal Article
• Authors: Das, Debayan; Namboodiri, S.
• Is Part Of: Chemical engineering science. Volume 229(2021)
• Publication Date: 2021-01-16
• Subjects: Whatman filter paper -- Nitrocellulose membrane -- Point-of-care -- LAMP -- Image analysis -- CFD studies; Chemical engineering -- Periodicals; Génie chimique -- Périodiques; Chemical engineering; Periodicals; Electronic journals; 660
• DOI: 10.1016/j.ces.2020.116130
• Languages: English
• ISSNs: 0009-2509

Abstract

Graphical abstract: Highlights: LAMP reaction is performed on NC and Filter paper membranes for E. coli detection. Dried LAMP reagents and detection dye are added to the membrane prior to DNA sample. Filter paper demonstrated uniform distribution of DNA amplicons across the surface. E. coli detection results are retained for a longer time on filter paper membranes. Extent and rate of LAMP amplification reaction is higher in Filter paper membranes. Abstract: Paper-based point-of-care (POC) devices have attracted tremendous attention because of low-cost, simple fabrication, and portability. LAMP-based POC platform comprises a paper substrate on which the DNA amplification reaction occurs. The quality of the paper substrate plays a critical role in performing an efficient LAMP reaction and for effective visualization of the amplification result. The major objective of the current work is to replace the frequently utilized nitrocellulose (NC) membrane by a cost-effective and high quality paper membrane viz. Whatman filter paper (WF) for rapid detection of E. coli via LAMP reaction. Isolated DNA is added on the paper membranes, which undergo LAMP reaction. The effect of initial DNA concentration and time on LAMP amplification reaction rate is analyzed on paper membranes via image analysis for the first time. Overall study inferred that WF is suitable for effective naked-eye detection and greater extent of LAMP reaction (generation of 19 968 DNA copies).